Sowing on the flora

The tank seeding on the flora and antibioticogram in gynecology are used to identify non-specific microflora in the material taken from the genital tract, to determine the potential pathogen of inflammation and its sensitivity to antibacterial drugs.

The study is performed using nutrient media. Sowing on the flora is carried out in favorable conditions for the development of microorganisms. Next, the results are evaluated. Thus, the identification of microorganisms and the study of their biological and biochemical properties are carried out.

Modeling of chemotherapeutic action of drugs outside the woman's body is carried out with the help of special tests that determine the sensitivity of the isolated microorganisms to bacteriophages, antiseptics and antibiotics. Thus, sowing on the flora allows you to select an effective medication for each specific case.

Analysis, having high specificity, has, at the same time, its own shortcomings. Due to the long term of the study, the increased requirements for the sampling process and the qualifications of the laboratory staff, the duration of treatment is increased. In clinical gynecology, planting on flora is used as an additional analysis in a complex of laboratory diagnostic measures.

Indications for the study are inflammation in the pelvic organs in women, including chronic or recurrent course, suspected of venereal diseases. Also, sowing on the flora is necessary for a comprehensive examination in order to establish the causes of miscarriage or infertility.

The collection of material in patients is performed before the beginning of anti-inflammatory therapy and menstruation. Before taking the analysis for fourteen days, stop taking any antibacterial drugs. At least one day you can not use vaginal suppositories, douche, and also have sexual intercourse. On the eve of the study, the use of a vaginal sensor is not recommended. For one or two hours, you should refrain from urinating, just before taking the test should not lead hygiene procedures on the external genitalia.

The test material should not contain impurities of blood. It should be taken before a manual gynecological examination.

The biological material is collected by a gynecologist when examined by vaginal mirrors, observing the rules of antiseptics. Typically, the analysis is taken from the vaginal vault (posterior), the urethra and the cervical canal. Before taking a swab from the cervical canal, the flora of the cervix is sterilized with saline. The mucous plug is pre-removed . Sampling of biomaterial samples is carried out with sterile cotton swabs, then they are placed in a sterile container. Labeled material is sent to a bacteriological laboratory in a short time. When removing the tampon, you should exclude its touching the walls of the vagina.

In the presence of bartholinitis, puncture of the Bartholin glands is performed . The contents of the abscess are removed with a sterile swab. When collecting material from the uterus, use a special tool like a syringe-aspirator. In the inflammatory process in the uterine appendages, a swab is taken during surgery or using the diagnostic puncture method through the vaginal vaults.

The results of the antibioticogram are determined by units of the minimum inhibitory concentration (MIC). To achieve the effect of therapy, it is necessary that the concentration of the drug in the patient's blood exceed the MIC by approximately four or eight times.